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Chronic Chagas disease: PCR-xenodiagnosis without previous microscopic observation is a useful tool to detect viable Trypanosoma cruzi Biol. Res.
Saavedra,Miguel; Zulantay,Inés; Apt,Werner; Martínez,Gabriela; Rojas,Antonio; Rodríguez,Jorge.
We evaluate the elimination of the microscopic stage of conventional xenodiagnosis (XD) to optimize the parasitological diagnosis of Trypanosoma cruzi in chronic Chagas disease. To this purpose we applied under informed consent two XD cages to 150 Chilean chronic chagasic patients. The fecal samples (FS) of the triatomines at 30, 60 and 90 days post feeding were divided into two parts: in one a microscopic search for mobile trypomastigote and/or epimastigote forms was performed. In the other part, DNA extraction-purification for PCR directed to the conserved region of kDNA minicircles of trypanosomes (PCR-XD), without previous microscopic observation was done. An XD was considered positive when at least one mobile T. cruzi parasite in any one of three...
Tipo: Journal article Palavras-chave: Chronic Chagas disease; PCR; Triatoma infestans; Trypanosoma cruzi; Xenodiagnosis.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300011
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Simultaneous effects of pH and substrate concentration on hydrogen production by acidogenic fermentation Electron. J. Biotechnol.
Cubillos,Genoveva; Arrué,Ramon; Jeison,David; Chamy,Rolando; Tapia,Estela; Rodríguez,Jorge; Ruiz-Filippi,Gonzalo.
The present research examined the effects of initial substrate concentration and pH on the yield and productivity of hydrogen production by acidogenic fermentation. Assays were carried out at three different initial pH levels (5.5, 6.5 and 7.5) and three initial substrate concentrations (3, 5 and 10 g COD/L). Glucose was used as carbon source and the experiments were conducted at 37°C in batch tests, after a thermal pretreatment to eliminate methanogenic microorganisms. Conversions of glucose into hydrogen were between 16.75 and 27.25% of theoretical maximum, and high values of hydrogen productivity were obtained. An optimum value for the yield of glucose between initial pH of 6.3 and 3.7 g COD/L and productivity of the 5.95 H2/gVSS h and initial pH of 6.7...
Tipo: Journal article Palavras-chave: Acidogenic; Gompertz modified; Heat treatment; Hydrogen; Surface response.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100011
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